Nonlinear Supra-Electroporation in Realistic Stem Cell Morphologies

Authors

  • Somen Baidya Department of Computer Science and Electrical Engineering University of Missouri–Kansas City Kansas City, MO, 64110, USA
  • Ahmed M. Hassan Department of Computer Science and Electrical Engineering University of Missouri–Kansas City Kansas City, MO, 64110, USA

Keywords:

Nonlinear, Stem Cells, Supra-Electroporation

Abstract

Exposing cells to ultrashort electric field pulses can lead to the permeabilization of the membranes of the internal cell organelles, such as the nucleus, which is termed as supra-electroporation. In this paper, we study the supraelectroporation of stem cells with realistic morphologies under nanosecond electrical pulse stimulation. For such short pulses, the cell and the nucleus membranes exhibit non-linearity in their conductance. Therefore, we used a non-linear model for the cells’ membranes, coupled with a quasi-static electromagnetic solver, and obtained the solution using a commercial Finite Element Method (FEM) solver. The results show that the outer shape of the cell has a strong effect on the magnitude and the spatiotemporal patterns of the electric field inside the cell, which affects the rate of the supra-electroporation of the nucleus. These variations in the rate of the supra-electroporation of the nucleus can guide the selective targeting of desired cells with specific shapes.

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References

L. Rems and D. Miklavčič, “Tutorial: Electroporation of cells in complex materials and tissue,” Journal of Applied Physics, vol. 119, no. 20, p. 201101, May 2016.

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K. C. Smith and J. C. Weaver, “Active mechanisms are needed to describe cell responses to submicrosecond, megavolt-per-meter pulses: cell models for ultrashort pulses,” Biophys. J., vol. 95, no. 4, pp. 1547-1563, Aug. 2008.

S. Baidya, A. M. Hassan, W. Al-Shaikhli, B. A. P. Betancourt, J. F. Douglas, and E. J. Garboczi, “Analysis of Different Computational Techniques for Calculating the Polarizability Tensors of Stem Cells With Realistic Three-Dimensional Morphologies,” IEEE Transactions on Biomedical Engineering, vol. 66, no. 7, pp. 1816-1831.

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Published

2020-11-07

How to Cite

Somen Baidya, & Ahmed M. Hassan. (2020). Nonlinear Supra-Electroporation in Realistic Stem Cell Morphologies. The Applied Computational Electromagnetics Society Journal (ACES), 35(11), 1326–1327. Retrieved from https://journals.riverpublishers.com/index.php/ACES/article/view/7523

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