Electric Field Distribution in a Biological Cell for Various Electrode Configurations-A Simulation Study
关键词:
Cell culture, dielectrophoresis, electrodes, HeLa cell摘要
Electroporation or electropermeabilization (EP) is a non-viral physical process of inducing and/or increasing permeability of biological membranes by the application of high intensity and short duration electrical pulses. The resulting high field strength in the membrane can lead to the formation of regions of increased permeability, often called pores that allow transmembrane transport of macromolecules, such as DNA and chemo drugs. The efficacy of EP depends on a number of parameters, such as electric field strength, duration, number of pulses, size of target cell, and type of drug or DNA to be fed, to mention a few parameters. While electric field strength, duration, and number of pulses are the dominant parameters for the electroporation effect, the electric field distribution also depends on the shape, the size, and the material of electrodes used. The various electrodes used in practice are the parallel plate electrodes, the needle electrodes, the needle array electrodes, and the caliper electrodes. Both six needle array and parallel plate electrodes were used in the skin cancer trials. The electric field distribution varies with the type of electrode. The choice of electrodes depends upon the type of application. For example, parallel plate electrodes produce good results in human clinical trials and the distance between the electrodes can be easily varied. Needle electrodes are the simplest, but due to their configuration, the field distribution is highly nonlinear; there is higher field strength around the needle tip. That is one of the reasons for using multiple needles or needle arrays to make the field distribution as uniform as possible. It is of practical interest to study the electric field distribution of various electrodes to make a more informed choice of which electrodes to use. This paper presents the results of a study of the electric field distribution in biological tissues for various electrode configurations. Magwel was used in this study. For the same voltage applied, the triangular and arc electrode configuration developed the highest electric field strength. Specifically, we develop simulation results that enhance or enable the acquisition of information from cells.
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