Characterization of Mesenchymal Stem Cells from Human Cortical Bone

Authors

  • Joseph S. Fernandez-Moure The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA
  • Bruna Corradetti Houston Methodist Research Institue, Houston, Texas, USA
  • Trevor Janecek The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA
  • Jeffrey Van Eps The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA
  • Matthew Burn The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA
  • Bradley K. Weiner The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA
  • Pranela Rameshwar Rutgers New Jersey Medical School, Newark, New Jersey, USA
  • Ennio Tasciotti Houston Methodist Research Institue, Houston, Texas, USA

DOI:

https://doi.org/10.13052/ijts2246-8765.2016.005

Keywords:

Mesenchymal stem cells (MSC), Human MSC

Abstract

Background Context: Mesenchymal stem cells (MSC) are being used for spine and orthopaedic surgical and research applications. Bone marrow and fat are the most commonly used sources of these cells.

Purpose: To describe a new technique allowing the isolation and expansion of human MSC from cortical bone.

Study Design: MSC from human cortical bone (vertebral lamina) were isolated, expanded, and verified in vitro.

Methods: Human MSC were isolated from laminar bone obtained during surgery (decompression/laminectomy). They were then cultured and assessed using fluorescence-activated cell sorting techniques for MSC markers, colony-forming unit assays, and multilineage differentiation.

Results: Isolated and cultured cells demonstrated MSC markers and trilineage differentiation confirming their stemness.

Conclusion: A novel method for the isolation of MSC from cortical bone has been described. These cells have significant current and future application in spine and orthopaedic surgery; and both the source of the cells and particular characteristics of the cortical bone derived MSC have advantages over currently used MSC obtained from other sources.

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Author Biographies

Joseph S. Fernandez-Moure, The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA

J. S. Fernandez-Moure M.D., M.S. is Chief Administrative Resident in Surgery at Houston Methodist Hospital in Houston, Texas. He graduated from college at Middlebury College in Middlebury, Vermont with degrees in Biochemistry and Spanish in 2002. Following this Dr. Fernandez-Moure went onto to the Rutgers University Graduate School of Biomedical Sciences in Newark, New Jersey to begin his Master’s in Science with a focus in molecular biology and stem cell biology. During this time he also enrolled in Rutgers New Jersey Medical School where he obtained his M.D. degree. Following this Dr. Fernandez-Moure was accepted into the residency in general surgery at Houston Methodist Hospital. Dr. Fernandez-Moure became the American Board of Surgery’s first surgeon scientist fellow and during that time was
promoted to Instructor in the Department of Nanomedicine.

Bruna Corradetti, Houston Methodist Research Institue, Houston, Texas, USA

B. Corradetti Ph.D., M.Sc. is currently an assistant professor at Universita Politecnica Delle Marche, Department of Life and Environmental Sciences in Ancona, Italy.

Trevor Janecek, The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA

T. Janecek, Houston Methodist. Leading Medicine. U.S. News & World Report has recognized Houston Methodist as one of the nation’s top 20 hospitals, placing it for the second time on the magazine’s prestigious Honor Roll. It is also designated as a Magnet hospital for excellence in nursing.

Jeffrey Van Eps, The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA

J. V. Eps M.D. is a senior resident physician in General Surgery at Houston Methodist Hospital and a Research Associate in the Center for Regenerative and Biomimetic Medicine at the Houston Methodist Research Institute.

Dr. Van Eps received his bachelor’s degree from Azusa Pacific University in southern California and his M.D. degree from Saint Louis University School of Medicine. He plans to pursue a career in academic subspecialty surgery and his research interests include unique applications of advanced biomaterial
matrices, controlled release mechanisms and autologous/synthetic factors as they relate to: augmentation of surgical wound healing, surgical analgesia, surgical device development, osteogenic and chondrogenic applications, and stem cell biology.

Matthew Burn, The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA

M. Burn M.D. is a Chief Resident in Orthopedic Surgery at Houston Methodist Hospital in Houston TX. Dr. Burns is a graduate of LSU School of Medicine in Shreveport.

Bradley K. Weiner, The Methodist Hospital Research Institute, Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA

B. K. Weiner is the Vice Chairman of Orthopaedic Surgery and the Chief of Spine Surgery at Houston Methodist. He has been at Houston Methodist since 2006 and serves as the Director for the Orthopaedic Surgery Residency Program. Dr. Weiner specializes in complex surgeries of the cervical, thoracic, and lumbar spines and he is recognized as a leader in spinal microsurgery.

Pranela Rameshwar, Rutgers New Jersey Medical School, Newark, New Jersey, USA

P. Rameshwar obtained her education at the University of Wisconsin-Madison, WI, USA and Rutger’s University, Newark, New Jersey, USA. Her main academic appointment is at Rutgers-New Jersey Medical School, Department of Medicine-Hematology/Oncology. Professor Rameshwar also has an appointment at the Graduate School of Biomedical Sciences. She is also a member of the joint doctoral program in biomedical engineering between University of Medicine and Dentistry of New Jersey and New Jersey Institute of New Jersey.

Ennio Tasciotti, Houston Methodist Research Institue, Houston, Texas, USA

E. Tasciotti Ph.D. is associate professor of Nanomedicine in the Institute for Academic Medicine at Houston Methodist Hospital in Houston, TX. Dr. Tasciotti earned his B.S. in Biological Sciences and his M.S. in Molecular Biology at Scuola Normale Superiore Pisa, Italy and a Ph.D. in Molecular Medicine from a joint program of the Scuola Normale Superiore Pisa with the International Center for Biotechnology and Genetic Engineering (ICGEB) in 2004. Dr. Tasciotti research as a Ph.D. student focused on gene and stem
cell therapy of cancer. He moved to the United States in 2006 as a senior postdoctoral fellow in the Department of Nanomedicine and Biomedical Engineering at the University of Texas Health Science Center at Houston. Following this appointment he transitioned to his current role of Director for the Center for Biomitmetic Medicine at Houston Methodist Hospital.

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Published

2016-11-21

How to Cite

Fernandez-Moure, J. S. ., Corradetti, B., Janecek, T., Eps, J. V., Burn, M., Weiner, B. K., Rameshwar, P., & Tasciotti, E. (2016). Characterization of Mesenchymal Stem Cells from Human Cortical Bone. International Journal of Translational Science, 2016, 71–86. https://doi.org/10.13052/ijts2246-8765.2016.005

Issue

Volume 2016

Section

Articles

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